Structural Investigation of Folic Acid by Nmr Proton Relaxation and Molecular Mechanics Analysis

Folic acid N-[4-(2-Amino-4-hydroxypteridinyl-(6)-methylamino)-benzoyl]-Laminoglutaric acid (Figure 1) is a fundamental coenzyme involved in onecarbon unit transfer processes. The solid state conformation of folic acid has been defined but there have been few investigations on the structure of this coenzyme in solution^. by Nuclear Magnetic Resonance (NMR). Information on dynamical motion, magnetic dipolar connectivities and energy minimization calculations are combined in order to define the solution structure of folic acid.